Abstract: This experiment examined the behavioral response of Desmognathus monticola to D. quadramaculatus, and specifically the effects of age, sympatry, and signal device on the response.  In the Desmognathus salamander system, D. quadramaculatus, the largest and most aquatic member of the genus, has been shown to push several of its sympatric congeners up the aquatic to terrestrial streambank gradient through predation. The impetus driving D. monticola, its next largest congener, is less clear, and appears to depend on the life history stage of the D. monticola.  Three age classes of D. monticola were investigated to detect any age-specific behaviors.  D. monticola naturally sympatric and allopatric to D. quadramaculatus were also compared to test for the ecotypic differences which may result from local adaptation.  Since chemical detection is suspected to be the mechanism D. monticola use in avoidance behaviors, the D. monticola in this experiment were introduced to both an actual D. quadramaculatus intruder and D. quadramaculatus-cued water.  Small experimental streambanks were used, and behavioral observations of the D. monticola were made before intruder introduction, immediately following introduction, and twenty four hours after introduction.  D. monticola distance from water, percent exposure, substrate choice, and number of seconds spent fleeing, under refuge, and frozen were recorded.  D. monticola moved farther from the water, shifted substrate preferences more terrestrially, and decreased percent exposure in the presence of the actual D. quadramaculatus intruder, but showed no significant response when given D. quadramaculatus-cued water.  D. monticola do exhibit a specific avoidance response to D. quadramaculatus, but chemical communication may not play a large role in this response.  No behavioral differences were detected across life history stages, perhaps due to experimental limitations.  Sympatric and allopatric D. monticola populations responded similarly to both types of D. quadramaculatus introduction, suggesting that gene flow may be occurring between the two populations.