Abstract: A majority of pathogens have the ability to infect more than one host, however it is often unknown whether they are true multihost pathogens or a number of related host-specific strains. Saprolegnia, a water mold belonging to the kingdom Chromista has been shown to infect a wide variety of organisms in fresh water environments and has the ability to act as both a pathogen and a saprophyte. The members of the genus Saprolegnia are known generalists and can be found growing on a wide variety of organisms. Saprolegnia is known to infect bullfrog (Rana catesbeiana) egg masses. This study compared Saprolegnia samples derived from various hosts within a fresh water pond based on their ability to infect and spread within a bullfrog egg mass. Through evaluating infectious and growth capabilities we addressed the level of variation and host specialization that can be found among Saprolegnia. I found that there was no variation dependent on strain for hyphal growth within an egg mass, but did find significant variation among strains for number of primary infections caused. This variation suggests a degree of specialization among strains of Saprolegnia within a pond. The fact that all strains were found to be at least mildly pathogenic to bullfrogs indicates that other organisms may play an indirect role as sources of Saprolegnia that are pathogenic to bullfrogs.