Abstract: The acidification of freshwater systems and its effect on aquatic organisms has been a major topic of study in the eastern United States in recent years. Anurans and pond-dwelling salamander species have been studied in-depth, but there is still much to learn about stream-dwelling salamanders. This study determined the LC50 for three species of stream-dwelling salamanders: Eurycea cirrigera, Desmognathus quadramaculatus, and Gyrinophilus porphyriticus, which were pH 3.46, 3.70, and 3.49, respectively. This study also examined the effects of stream buffering capacity and species identity on LC50 levels. We found that stream buffering capacity does not have an effect on the LC50 levels of the three stream salamanders in this study. The LC50 levels for the salamanders in this study were relatively low when compared to other amphibian LC50 levels. After reviewing water chemistry data available for the state of Virginia, we feel that these salamanders are not in immediate danger due to acidification.